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The aim of this study is to evaluate the preliminary phytochemical screening and anti-anxiety activity of the ethanolic extract of areal parts of Rhusmysorensisin rat models. The ethanolic extract of Rhusmysorensiswas prepared using 97% ethanol 72hrs by maceration. The oral dose of 200 and 400 mg/kg of the extract was evaluated for the activity in elevated plus maze and open field models. The obtained results have shown that the Rhusmysorensisethanolic extract shown the presence of flavonoids, alkaloids, terpenoids, tannins and steroids and also shown the significant anti-anxiety activity on wistarrats on dose dependent manner. based on the results it is concluded that the ethanolic extract of areal parts of Rhusmysorensisis having significant anti-anxiety activity on wistar rats. Further investigation is required by using the purified compound to evaluate and isolation of the phytochemicals responsible for the anti-anxiety activity of ethanolic extract of Rhusmysorensis.