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Abstract
The possible protective effect of ethanolic extract of P.retticultus leaves (EEPR) on diabetes and diabetes-induced oxidative stress was evaluated in Streptozotocin (STZ)-induced diabetic male adult wistar albino rats.. Experimental animals were divided into five groups viz., group-1 control normal saline, group-2 diabetic control, group-3 test dose for 200mg, group-4 test EEPR for 200mg/kg body weight,p.o, group-5 standard dose of glibenclamide0.5mg/kg,b.w.p.o. Diabetes mellitus (DM) was induced in groups II and III mice by a single intraperitoneal injection of Streptozotocin (50 mg/kg body wt). Group I (control mice) received an equal volume of normal saline. Group III mice were further treated with EEPR (200 mg/kg body wt, p.o.) for a period of 21 days. Body weight and fasting blood glucose (FBG) levels were measured at periodic intervals during the test period. At the end of treatment period, blood was collected by cardiac puncture under mild ether theo pental sodium and serum was isolated to analyze its lipid profile i.e. serum total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL) and very low density lipoprotein (VLDL). The homogenates of hepatic, pancreatic and renal tissues were also analyzed for both enzymatic and non-enzymatic antioxidants, such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), reduced glutathione (GSH), thiobarbituric acid reactive substances (TBARS) and total protein (TP).