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Abstract
Diospyros lotus Fruits is a well-known antidiabetic medicinal plant used for several traditional medicine aspects in different areas of the world, including India. This study includes phytochemical analysis, antidiabetic evaluation. The Fruits of Diospyros lotus has been used in traditional health systems to treat diabetes mellitus in India. However, the antidiabetic activity of this medicinal plant is not scientifically validated and authenticated. The present study aimed to investigate the in vitro and in vivo anti-diabetic activity of Fruits crude extract and solvent fractions of Diospyros lotus. The aim of this study was to investigate the antidiabetic activity and mechanism of action of Ethanolic extract prepared from Diospyros lotus Fruits. The result of α–amylase enzyme inhibition activity was found in a dose-dependent manner, the strongest activity was shown by Crude extract fraction (89.60% inhibition at 1000 μg/mL) compared to the standard acarbose having 97.19% inhibition at 1000 μg/mL. The crude extract of Diospyros lotus Fruits showed significant blood glucose-lowering effect on hypoglycemic rats and oral glucose loaded rats. In Streptozotocin-induced diabetic rats model, the crude extract fraction significantly decreased the fasting blood glucose level after 14 days of treatment. The result demonstrated the beneficial biochemical effects of Diospyros lotus Fruits extract by inhibiting α–amylase improving serum lipid profile levels. The leaves crude extract are effective in lowering blood glucose levels in diabetic and hypoglycemic rats. The claimed traditional use as antidiabetic has scientific ground.