Main Article Content
Abstract
A simple, specific, accurate, precise and stability indicating RP-HPLC method was developed and validated for the simultaneous estimation of Desvenlafaxine and Saxagliptin in pharmaceutical formulation. The method was developed using Buffer (pH2.16):1.0mLofortho phosphoric acid, 85%solution was transferred into a 1000 mL of volumetric flask. The volume was made up with water and mixed well. Mobile Phase: The above buffer solution(pH2.16) was mixed with HPLC grade acetonitrile in a ratio of 50:50V/V and degassed. Optimised conditions of developed method was found at retention time of Desvenlafaxine is 2.52 & Saxagliptin is 3.51, The proposed method was validated for linearity, range, accuracy, precision, robustness, LOD and LOQ. The proposed method was validated for linearity, range, accuracy, precision, robustness, LOD and LOQ. Linearity was observed over a concentration range 0.9999 for Desvenlafaxine and 0.9999 for Saxagliptin. The % RSD for Intraday and Inter day precision was found to be 0.35and 0.21 for Desvenlafaxine and 0.68and 0.49 for Saxagliptin. The LOD and LOQ were found to be 1.008and 3.050 for Desvenlafaxine and LOD and LOQ were found to be 0.244 and 0.740 for Saxagliptin respectively. Desvenlafaxine and Saxagliptin were subjected to stress conditions of degradation including acidic, alkaline, oxidative, thermal and photolysis.