Main Article Content

Abstract

A Simple, sensitive, specific and precise RP-HPLC method for the pharmaceutical dose estimation of Vismodegib. Chromatogram was run through AscentisC18 150 x 4.6 mm, 5m. Mobile phase containing 0.1%NA2HPO4: Acetonitrile taken in the ratio 60:40was pumped through column at a flow rate of 1.0ml/min. Buffer used in this method was Sodium Hydrogen Phosphate (4.0ph) buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 260.0nm. Retention time of Vismodegib was found to be 2.585 min. %RSD of the Vismodegib were and found to be 0.9. %RSD of Method precision of Vismodegib was found to be 0.5.%Recovery was obtained as 99.76% for Vismodegib. LOD, LOQ values obtained from regression equation of Vismodegib were 0.57, 1.72. Regression equation of Vismodegib isy = 37710x + 19681. Retention times were decreased, and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.

Keywords

Vismodegib RP-HPLC Dosage form Formulation

Article Details