EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF LILIUM CANDIUM.L IN ANIMAL MODELS

Abstract

Lilium Candium (Family liliaceae) commonly known as Madonna lilies is a vigorously growing in India. In the present study a pharmacognostic evaluation of the leaves was undertaken. In addition to the evaluation of physicochemical characteristics; preliminary Phytochemical parameters and pharmacological activities of aqueous extracts has been carried out. The aim of the present study was carried out with the objective of phytochemical screening and to evaluate the hepatoprotective activity of aqueous extract of L.candium.
Liver is the largest organ in the body which serves as a gland also. It plays an important role in the maintenance of internal environment through its multiple and diverse functions. Any damage to the liver or impairment of its functions leads to injurious effects. Liver diseases (like jaundice) are the common ailments affecting mankind, though no remedy is available in allopathic at present. In the recent past years many medicinal plants are screened for their hepatoprotective activity and quite a few of them are already successful in entering the market, Hence the present study is planned to find out the hepatoprotective activity of Lilium Candium using drug induced hepatotoxicity models like Paracetamol, CCL4 and Acetamenophenon induced methods .
The rats were divided into five groups with six rats in each for three models. Group I (Control) served as normal and received the vehicle alone (Sterile distilled water, 10 ml/kg, p.o.) for 5 days. Group II (Toxin control) animals on the 3rd and 4th day. Group III and IV were treated with LCM at a dose level of 400 mg/kg and 600 mg/kg body weight p.o. per day respectively for 5 days and on the 3rd and 4th day with hepatotoxic drugs was given 1 h after the treatment of the extract. Group V (Standard) was treated with standard drug silymarin (100 mg/kg p.o.) for 5 days and on the 3rd and 4th day hepatotoxic drugs was given 1h after the treatment of the drug. The animals were sacrificed 48 h after the last injection of hepatotoxic drugs under mild ether anesthesia. The blood was collected and allowed to stand for 30 min at 37°C and then centrifuged to separate the serum to estimate various biochemical parameters.
In hepatoprotective studies, the induced Diclofenac, CCL4 and Acetamenophenon toxicity elevated levels of serum marker enzymes ALT, AST, ALP and the level of BUN along with the decrease in total protein and albumin levels. It also increased the relative liver weight and decreased the level of liver total protein and GSH. The activity of catalase and GPx significantly decreased in diclofenac intoxicated animals. The pre-treatment of methanol extract of Lilium candidum at dose levels of 400 and 600 mg/kg had restored the ALT, AST, ALP and BUN levels towards normalization and the effects were comparable with standard drug (Silymarin 100 mg/kg). The total protein, albumin, GSH levels and catalase, GPx activity increased significantly in the animals received pre-treatment of the LCM.
The data obtained from animal experiments are expressed as mean ± SEM (standard error of mean). For statistical analysis data were subjected to analysis of variance (ANOVA) followed by Student’s t-test. Values are considered statistically significant at p < 0.01 for ANOVA and P < 0.05 for t-test.