Development and Evaluation of Allamanda cathartica Leaf Ointment for Wound Healing Potential

Abstract

The objective of the present investigation was to study the wound healing activity of petroleum ether extract of Allamanda cathartica Leaves ointments in animal model. Allamanda cathartica belongs to the family Apocyanaceae. The prepared extract was subjected to phytochemical screening to determine the presence of various phytoconstituents. Petroleum ether extract of Allamanda cathartica (AC) was subjected to formulation of Ointments consisting various bases viz., oleaginous base, Absorption base, Hydrophilic base and PEG bases by traditional incorporation method. Owing to all the results of physical parameters characterization, Ointment formulations of AC extract containing Hydrophilic base (OF3, OF7) and PEG base (OF4, OF8) were subjected to in vitro drug diffusion studies. OF3 and OF4 formulations comprising low concentration (5%) of Hydrophilic and PEG bases have shown good dissolution characters by releasing 90.76% and 85.43% active principle. There was no evidence of phase separation, development of disagreeable odour, change in colour, consistency and no change in parameters like pH, Viscosity, Spreadability of the all the products during stability study for three months. In the in vivo study the topical application of ACHB and ACPEG of petroleum ether extract ointments on the infected wound of the rats caused a significant (P<0.05) and faster rate of wound closure and reduced the epithilization period.