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Abstract
Pisonia alba leaves have been traditionally used for many ailments including cancer. In the present study, anti-cancer activity of ethanolic leaf extract of Pisonia alba (EEPA) was evaluated using both in vitro and in vivo methods. EEPA was subjected to preliminary qualitative phytochemical investigations by using standard procedures. In vitro antitumor activity of EEPA was evaluated by the MTT assay method using The human cervical cancer cell line (HeLa). Then the extract subjected to in vivo anti cancer activity using Dalton's Lymphoma Ascites (DLA) tumor model. The activity was assessed Increase in life span, average increase in body weight, changes in food intake, tumor volume, tumor weight, viable cell count, non viable cell count, PCV, Total cell count and hematological studies. The potency of the extract was compared with standard 5-flurouracil (20 mg/kg i.p.).In in vitro anti cancer activity EEPA exhibited significant cytotoxic activity against both cell lines even at different concentrations. Oral administration of EEPA at the dose of 200 and 400 mg/Kg, significantly (p < 0.001) increased the survival time, non viable cell count and decreased the average body weight and food intake, viable cell count of the tumor bearing mice. After 14 days of inoculation, EEPA was able to reverse the changes in the hematological parameters, protein and PCV consequent to tumor inoculation.The results indicate that EEPA possess significant antitumor activity on dose dependent manner.