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Abstract

Objectives

The aims of this research were to carry out the preliminary phytochemical screening and antioxidant activity of different extracts of J. sessiliflorum. The different anti-oxidant methods carried out were DPPH scavenging method, NBT dye reduction method and nitric oxide scavenging method


Methods

Extracts were prepared by reflux method using different polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant activities using DPPH, NBT dye reduction method and nitric oxide scavenging methods and the correlation of their IC50 values with standards were carried out.


Results

The ethanolic herbs extract of J. sessiliflorum had the lowest IC50 values in all the anti-oxidant methods. Moreover, the ethanolic extracts showed the presence greatest amount of phytochemical constituents. The IC50 values were correlated with the IC50 values of standards in all the anti- oxidant activity determination methods.


Conclusions

The results of the present study indicate that the extracts of J.sessiliflorum exhibited strong antioxidant activity and thus it is a good source of antioxidant.

Keywords

Antioxidant DPPH NBT dye reduction Nitric oxide scavenging J.sessiliflorum

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