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Abstract
Microbial exopolysaccharides are remarkable polymers created by living organisms that protect them from external factors. Following the appearance of a fermentative approach, these polymers are industrially collected from the medium cultures. These materials are biocompatible and biodegradable, with precise and advantageous architectures for biomedical drug delivery, as well as the ability to self-assemble. The use and modification of exopolysaccharides as future low-cost resources for the creation of valuable antioxidant and anticancer tablets. The influence of several types of media on the synthesis of EPS and the growth of Bacillus subtilis was investigated in this study. The highest growth of B.subtilis was observed on solid basal media. EPS production increased during the culture's growth period, with the peak occurring after 72 hours. Bacillus subtilis produced the most growth when the pH was set to 7.0. Sucrose was suitable to be a viable carbon source since it boosted the formation of EPS. The polysaccharides C-O-C and C-O-P were absorbed at 1037 cmG1, while glucose in pyranose form was absorbed at 1000 cmG1. Polysaccharides revealed the clear typical absorption at 1037 cmG1 in the anomeric region (1000-1600 cmG1). Bacillus subtilis exopolysaccharides were studied and prepared, as well as their applications in pharmaceuticals, and more research is needed to analyze and access Exopolysaccharides from Bacillus subtilis.