International Journal of Pharmacology and Clinical Research (IJPCR)

Reverse Phase-High Performance Liquid Chromatography Method for the Simultaneous Estimation of Trifluridine and Tipiracil in Pure Form and Tablet Dosage Form

2022-11-01T15:06:14+00:00

A new, simple, precise, rapid, selective and stability reversed-phase high performance liquid chromatographic (RP-HPLC) method has been developed and validated for the simultaneous quantification of Trifluridine and Tipiracil in pure form and its pharmaceutical dosage form. The method is based on Phenomenex Gemini C18 (4.6×250mm) 5µ column. The separation is achieved using isocratic elution by Methanol: TEA Buffer in the ratio of 65:35% v/v, pumped at flow rate 1.0mL/min and UV detection at 230nm. The column is maintained at 40°C throughout the analysis. The total run time is about 6min. The method is validated for specificity, accuracy, precision and linearity, robustness and ruggedness, system suitability, limit of detection and limit of quantitation as per International conference of harmonization (ICH) Guidelines. The method is accurate and linear for quantification of Trifluridine, Tipiracil between 10 - 50µg/mL and 20 - 100µg/mL respectively. Further, satisfactory results are also established in terms of mean percent- age recovery (100.37% for Trifluridine and 100.34% for Tipiracil, intra-day and inter-day precision (<2%) and robustness. The advantages of this method are good resolution with sharper peaks and sufficient precision. The results indicate that the method is suitable for the routine quality control testing of marketed tablet formulations.

Evaluation of anti hyperlipedimic activity of hibiscus cannabinus extract in experimental animal model

2022-11-10T10:20:17+00:00

To investigate the anti Hyperlipidemic activity of methanol extract of Pterocarpus marsupium s in male Wistar rats. In this model of Hyperlipidemia, 30 adult male wistar rats (150-200gms) were evenly divided into 5 groups in both groups. Group-1 and Group-2 served as untreated and model controls respectively, while Group-3, 4 and 5 were the treatments groups which were simultaneously treated with standard, 200 and 400 mg/kg extract respectively along with High Fat Diet and Triton x 100. On last day, blood samples for biochemical parameters, were obtained under inhaled diether anaesthesia. In the model of anti diabetic animals were evenly divided into 5 groups. Group-1 and Group-2 served as untreated and model controls respectively, while Group-3, 4 and 5 were the treatments groups which were simultaneously treated with standard, 200 and 400 mg/kg extract respectively after glucose loading. HFD and Triton x 100 treatment caused Hyperlipidemia as evidenced by marked elevation in Cholesterol, Triglycerides, LDL, VLDL and decrease in HDL levels. Co-administration of extract with HFD and Triton x 100 decreased rise Cholesterol, Triglycerides, LDL, VLDL and increase in HDL levels. It was observed that the methanol extract of Pterocarpus marsupium conferred Anti- Hyperlipidemia activity by biochemical observation against HFD and Triton-x-100 induced Hyperlipidemia in rats. In the near future could constitute a lead to discovery of a novel drug for treatment of drug induced Hyperlipidemia.

Nephro protective activity of plumbago zeylanica extract on gentamicin induced nephrotoxicity in rats

2022-11-10T10:36:53+00:00

Herbal medicine is the oldest form of healthcare known to mankind and most cultures have long folk medicine histories that include the use of plants. Nephrotoxicity is one of the most common kidney problems and occurs when the body is exposed to a drug or toxin that causes damage to the kidneys. To investigate the Nephroprotective activity of ethanol extract of Plumbago zeylanica on Gentamicin induced Nephrotoxicity in male Wistar rats. In this model of Nephrotoxicity, 30 adult male wistar rats (150-200gms) were evenly divided into 5 groups. Group-1 and Group-2 served as untreated and model controls respectively, while Group-3, 4 and 5 were the treatments groups which were simultaneously treated with standard, 200 and 400 mg/kg extract respectively, after each dose Gentamicin (80 mg/kg, i.p.) for 10 day. On 11^th day, blood samples for biochemical parameters, while the rats kidneys for histology were obtained under inhaled diether anaesthesia. Gentamicin treatment caused Nephrotoxicity as evidenced by marked elevation in blood urea, uric acid and Creatinine. Co-administration of extract with Plumbago zeylanica decreased rise in blood urea, uric acid and Creatinine. Apart from these, histopathological changes also showed the protective nature of extract against Gentamicin induced necrotic damage of renal tissues. It was observed that the ethanol extract of conferred nephroprotective activity by histopathological and biochemical observation against Gentamicin induced Nephrotoxicity in rats. In the near future could constitute a lead to discovery of a novel drug for treatment of drug induced Nephrotoxicity.