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Yellow fever is a viral infection spread by a certain type of mosquito that is the yellow fever mosquito Aedesaegypti and this viral disease is typically short duration. Yellow fever is common in tropical and subtropical areas of South America and South Africa. The actual caused of yellow fever is done by yellow fever virus, that belongs to the family of Flaviviridae. Sign and symptoms of yellow fever generally carried cause a mild infection with fever, headache, back pain, fatigue, chills, muscle pain, loss of appetite, nausea and vomiting. The transmission of yellow fever virus is mainly transmitted through the bite of the yellow fever mosquito Aedesaegypti. Diagnosis of yellow fever is generally performed by laboratory testing of serum to detect virus specific IgM and neutralizing antibodies. Prevention of yellow fever includes vaccination, try to stay away from mosquitoes and also avoidance of mosquito bites in areas where yellow fever is endemic. For the treatment of yellow fever there is no specific treatment like vaccine, antidote or antiviral medications etc. During the early stages of yellow fever the diagnosis is difficult but generally diagnosis of yellow fever is done by laboratory testing of Serum to detect virus-specific IgM and neutralizing antibodies.